Table 2 Plasmids used in this study
From: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
Name | Origin of replication | Antibiotic marker | Description | Reference |
|---|---|---|---|---|
pJBEI-6409 | p15A | Chloramphenicol | Limonene from acetyl-CoA via GPP. Plasmid map- UV5prom-AtoB-saHMGS-saHMGR-T1ter-pTrc-MK-PMK-PMD-idi-B0015term-pTrc-trGPPS-tLS-p15Aori-camR-lacI | pJBEI-64093 |
pJBEI-3101 | ColE1 | Ampicillin | Limonene from IPP and DMAPP via GPP. Plasmid map-pTrc-trGPPS-tLS-t1ter-ampR-ColE1-lacI | pJBEI-31013 |
EcoCTs-CPMS5 | ColE1 | Ampicillin | Plasmid for overexpression of limonene synthase. pJBEI-3101-del-trGPPS. | This study |
EcoCTs-CPMS5-glk | ColE1 | Ampicillin | Plasmid for overexpression of glucokinase | This study |
pCas-V5 | repA101 | Kanamycin | Plasmid to express cas9 under its native promoter, λRed from pBAD promoter. | |
pTargetT-pJ23119_adhE-p-sacB | pMB1 | Spectinomycin | Plasmid expresses gRNA, SacB under sucrose inducible promoter and harbours the homologous arm. | |
pTargetT-pJ23119_ldhA-p-sacB | pMB1 | Spectinomycin | Plasmid expresses gRNA, SacB under sucrose inducible promoter and harbours the homologous arm. |
