Fig. 1: NHE9 associates with an EMTed phenotype.
From: Endosomal pH is an evolutionarily conserved driver of phenotypic plasticity in colorectal cancer
a Volcano plot depicting the association of NHE9 levels with phenotypic parameters that drive cancer evolution based on the NCI-60 database. b Gene effect scores for the NHE9 and NHE6 from CRISPR knockout screens in colorectal cancer (CRC) cell lines. Scores were normalised so that nonessential and essential genes had median scores of 0 and -1 across cell lines, respectively. c Mutation frequencies of NHE9 and other cancer drivers in each of the hypermutated CRC samples from the TCGA database. For each tumour, microsatellite instability (MSI) status, MLH1 silencing, as well as mutational status are indicated as per the legend above. d Lollipop plot of NHE9 mutations identified in hypermutated CRC samples. The x-axis represents the amino acid positions of NHE9. e Side (left) and top (right) views of the dimer of the NHE9 transport domain, coloured according to the degree of ConSurf conservation, with green to purple denoting variable to conserved amino acid positions. NHE9 mutations found in hypermutated CRC are shown as alpha-carbon spheres. The conservation colour bar is displayed at the bottom. f NHE9 expression in CRC relative to healthy control samples (GSE4107) represented as scatter plots displaying mean ± SD. g Overview of all three EMT scoring metrics. The illustration was created with BioRender. h Scatter plots depicting the relationship between NHE9 expression and all three EMT scores in CRC and healthy control samples (GSE4107). P values were calculated by Pearson’s correlation (a, h) and unpaired, two-tailed t test (f). See related Supplementary Fig. 1.
