Fig. 3: NHE9 high tumours have high migration levels.
From: Endosomal pH is an evolutionarily conserved driver of phenotypic plasticity in colorectal cancer
a Scatter plots depicting the relationship between NHE9 expression and EMT scores (KS) in CRC samples from the GSE16125 (n = 36) (left), GSE28722 (n = 125) (centre), and TCGA (n = 637) (right) databases. Each plot shows the linear fit and 95% confidence interval, Pearson’s correlation coefficient (R), and P-value. b Pairwise Pearson’s correlation between NHE9 and EMT regulatory genes in CRC samples from the GSE16125 (left), GSE28722 (centre), and TCGA (right) databases. Pearson’s correlation value for each gene pair is represented by the size of the circle, filled with the corresponding colour from the colour bar at the bottom. *P < 0.05, **P < 0.01, ***P < 0.001. c Hierarchical clustering and heat map (from low (blue) to high (red)) of the activity levels (ssGSEA scores) of three independent migration gene sets as well as partial EMT (pEMT), EMT, cAMP inhibited, and cAMP activated gene sets in CRC samples from the GSE16125 (top left), GSE28722 (top right), and TCGA (bottom) databases. Note enrichment of migration signatures in tumours with higher (NHE9hi) versus lower (NHE9lo) NHE9 expression levels. See related Supplementary Figs. 3–5.
