Fig. 4: Single cell analysis of NHE9 expression in colorectal cancer.

a Bar graph depicting the percentage of epithelial cells classified as CMS1, CMS2, CMS3, and CMS4 subtypes. b Scatter plot depicting the CMS subtype-wise association between EPI and MES signatures. c–e Scatter plot depicting the CMS subtype-wise association between EPI signatures and 76GS (c), MLR (d), and KS (e) EMT scores. f Bar graph depicting heterogeneity in NHE9 expression among CMS subtypes. Note significant high expression of NHE9 in CMS4 than other subtypes. The error bars reflect standard deviation. g Pairwise Spearman correlation of NHE9 with EPI and MES signatures and EMT scores (76GS, MLR, and KS) in CMS4 cells. Correlation value for each comparison pair is represented by the size of the circle, filled with the corresponding colour from the colour bar at the bottom. *P < 0.05, **P < 0.01, ***P < 0.001. h Pairwise Spearman correlation of NHE9 with three independent migration gene sets (GO cell migration, Wu cell migration, and circulating tumour cell or CTC) as well as partial EMT (pEMT), EMT, cAMP inhibited, and cAMP activated gene sets in CMS4 cells. Correlation value for each comparison pair is represented by the size of the circle, filled with the corresponding colour from the colour bar at the bottom. *P < 0.05, **P < 0.01, ***P < 0.001. The analysis was performed on the GSE13257 dataset. P values were calculated by Spearman correlation (b–e, g, h) and one-way ANOVA (f).