Fig. 6: Evaluation of the effects of ETS-1 modulation on dopamine production function.

Using iPSC-derived midbrain neurons, we evaluated the effects of ETS-1 perturbation on cell viability and the expression of TH, the rate-limiting enzyme in dopamine production. For real-time quantitative PCR, the mRNA expression of each gene were normalized to that of GAPDH, and the expression of the control group was set to 1. For cell viability measurements, the luminescence values were converted to ATP levels and normalized by setting the viability of the control group to 100%. A The differentiation of iPSC-derived midbrain neurons was confirmed by immunostaining. TH (green) was stained as a midbrain marker, while nucleus was visualized by Hoechst (blue dots). The white bar indicates 100 µm. B ETS-1 mRNA expression in midbrain neurons overexpressing ETS-1 (n = 3). C ETS-1 mRNA expression in midbrain neurons with suppressed endogenous ETS-1 expression (n = 3). D Viability of midbrain neurons overexpressing ETS-1 (n = 4). E Viability of midbrain neurons with suppressed endogenous ETS-1 expression (n = 4). F TH mRNA expression in midbrain neurons overexpressing ETS-1 (n = 3). G TH mRNA expression in midbrain neurons with suppressed endogenous ETS-1 expression (n = 3). Data are presented as Mean ± SD, *p < 0.05, **p < 0.01, N.S. not significant versus control.