Fig. 7: Evaluation of the effects of ETS-1 modulation on dopamine receptor function.

Using iPSC-derived forebrain neurons, we evaluated the effects of ETS-1 modulation on dopamine receptor function. For real-time quantitative PCR, the mRNA expression of each gene was normalized to that of GAPDH, and the expression in the control group was set to 1. In the cell viability assay, luminescence values were converted to ATP levels and normalized by setting the viability of the control group to 100%. A The differentiation of iPSC-derived forebrain neurons was confirmed by immunostaining. GABA (green) was stained as a midbrain marker, while nucleus was visualized by Hoechst (blue dots). The white bar indicates 100 µm. B ETS-1 mRNA expression in forebrain neurons overexpressing ETS-1 (n = 3). C ETS-1 mRNA expression in forebrain neurons with suppression of endogenous ETS-1 expression (n = 3). D DRD1 mRNA expression in forebrain neurons overexpressing ETS-1 (n = 4). E DRD1 mRNA expression in forebrain neurons with endogenous ETS-1 suppression (n = 3). F DRD2 mRNA expression in forebrain neurons overexpressing ETS-1 (n = 4). G DRD2 mRNA expression in forebrain neurons with suppression of endogenous ETS-1 expression (n = 3). H Viability of forebrain neurons overexpressing ETS-1 (n = 4). I Viability of forebrain neurons after suppression of endogenous ETS-1 expression (n = 3). J Number of DRD1-positive cells in forebrain neurons overexpressing ETS-1. Data are presented as Mean ± SD, *p < 0.05, **p < 0.01, N.S. not significant versus control.