Fig. 2
Cellular immune response in vaccinated mice. Proliferative dose-response curve of spleen cells harvested 30 dpi from vaccinated mice upon increasing concentrations of Traspain. Proliferation index (PI) (a). Secreted cytokines by ELISPOT assay at 30 dpi. Representative ELISPOT plate showing IFN-γ producing colonies of spleen cells from indicated groups cultivated in the presence of 10 µg/ml of Traspain (b). Pooled-splenocytes were re-stimulated with the indicated protein and mean number of spot forming units were determined for IFN-γ, IL-17, IL-2, or IL-4 (c). Results are expressed as mean ± SEM, n = 18 from 6 mice per group. One-way-ANOVA+Dunnett multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 comparing with c-di-AMP group. Bars indicate significant difference (p < 0.05) between the indicated category of stimulus and groups, two-way-ANOVA + Tukey’s multiple comparisons test. All results are representative of two independent experiments
