Table 1 Binding capacity of sera from Pf rCSP-CAF09-immunized mice to P. falciparum and P.b.-P.f. CSP-FL CD8CT transgenic sporozoites

From: Robust antibody and CD8+ T-cell responses induced by P. falciparum CSP adsorbed to cationic liposomal adjuvant CAF09 confer sterilizing immunity against experimental rodent malaria infection

Immunization

Qualitative score of tested sera—P. falciparum sporozoites

 

1:1000

1:4000

1:16000

1:64000

1:256000

1:1024000

Pf rCSP-CAF09 2×

+++

+++

++

++

+

−

Pf rCSP-CAF09 3×

+++

+++

+++

++

+

+/−

CAF09 3×

−

−

−

−

−

−

Immunization

Qualitative score of tested sera—P.b.-P.f. CSP-FL CD8CT sporozoites

 

1:1000

1:4000

1:16000

1:64000

1:256000

1:1024000

Pf rCSP-CAF09 2×

+++

+++

++

++

+

−

Pf rCSP-CAF09 3×

+++

+++

++

++

+

+/−

CAF09 3×

−

−

−

−

−

−

  1. Pooled serum samples from mice immunized two or three times (n = 6/group) with Pf rCSP-CAF09 were incubated on slides coated with fixed sporozoites and stained with fluorescently labeled anti-mouse secondary antibody. Slides were qualitatively scored under a microscope
  2. +++, strong;++, good;+, weak; and − no reactivity
  3. Pf rCSP-CAF09, P. falciparum recombinant circumsporozoite protein in adjuvant CAF09; P.b.-P.f. CSP-FL CD8CT, P. berghei–P. falciparum CSP full-length CD8 epitope C-terminus transgenic parasite
Back to article page