Table 4 Rationale for construction of immunogenic preparation: Predicted binding affinity of worldwide octamer/nonamers
From: Protein nanovaccine confers robust immunity against Toxoplasma
Predicted IC50 nM | Stability prediction | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Pair | Origin | Peptide | Length | Median | ANN | SMM | NetMHCpan | Pred | Thalf(h) | %Rank_Stab |
1 | SAG2C (13–21) | STFWPCLLR | 9 | 13 | 13 | 19 | 10 | 0.846 | 4.15 | 0.50 |
1 | SMFWPCLLR | 9 | 18 | 17 | 40 | 18 | 0.309 | 0.59 | 4.00 | |
2 | SRS52A (250–258) | SSAHVFSVK | 9 | 14 | 14 | 18 | 7 | 0.806 | 3.22 | 0.70 |
2 | SSAYVFSVK | 9 | 13 | 13 | 19 | 8 | 0.739 | 2.30 | 0.90 | |
3 | GRA5 (89–98) | AVVSLLRLLK | 10 | 17 | 18 | 15 | 17 | 0.948 | 12.86 | 0.12 |
3 | AVVSLLRLLR | 10 | 128 | 81 | 128 | 132 | 0.730 | 2.20 | 1.00 | |
