Fig. 1 | npj Vaccines

Fig. 1

From: Newcastle disease virus-based H5 influenza vaccine protects chickens from lethal challenge with a highly pathogenic H5N2 avian influenza virus

Fig. 1

Schematic design of recombinant NDV-H5 constructs and characterization of recombinant NDV-H5 virus in infected Vero cells. a The chicken codon-optimized ectodomain of hemagglutinin gene from the A/chicken/Iowa/04-20/2015 (H5N2) was fused with the transmembrane and cytoplasmic tail of fusion (F) protein of NDV-LaSota strain, and cloned into the P and M junction of the NDV antigenomic cDNA. The H5 ectodomain was placed under the control of a set of NDV gene start (GS) and gene end (GE) transcription signals directing its expression as a separate mRNA. b, c H5 protein was expressed and detected in NDV-H5 virus infected Vero cells using an HA human-monoclonal antibody. NDV proteins were detected in Vero cells infected with the NDV-H5 or NDV LaSota virus using mouse polyclonal NDV serum by immunofluorescence and flow cytometry assays

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