Fig. 1

Glycosylation of the extended polysaccharide chain. a A plasmid co-expressing CTB4573H and PglL was transformed into S. Paratyphi A waaL/cld double mutant strain 50973DWC. Western blot assays were conducted to analyze glycosylation of CTB4573H with or without CldLT2 in 50973DWC. Samples were derived from the same experiment. b Western blot analysis of glycosylated rEPA4573H with the extended polysaccharide chain co-expressing CldLT2, rEPA4573H, and PglL. Samples were derived from the same experiment and gels/blots were processed in parallel