Table 1 Main plasmids used in this study
Plasmids | Characteristic | Source |
|---|---|---|
pKD3 | Template plasmid for chloramphenicol resistance fragment flanked by FRT (FLP recognition target) sites | Laboratory stock |
pKD46 | used for λ-red recombination, araC-ParaB, ApR | Laboratory stock |
pCP20 | Used for the removal of chloramphenicol gene | Laboratory stock |
pACU184-cldLT2 | Encodes Cld from S. typhimurium and under control of Tac promoter, CmR | This work |
pET-pglL-rEPA4573H | Encodes PglL and 6×His-tagged rEPA4573, both of them are under control of Tac promoter, KanR | Laboratory stock |
pET-pglL-CTB4573H | Encodes PglL and 6×His-tagged CTB4573, both of them are under control of Tac promoter, KanR | Laboratory stock |
pET-pglL-CTB4573 | Similar with pET-pglL-CTB4573 but have no 6×His-tag | This work |
pET-pglL-CTB4563P2H | Encodes PglL and 6×His-tagged CTB4563P2, both of them are under control of Tac promoter, KanR | This work |
