Fig. 5
PCA and correlation analysis of the clinical, virological and immune response data of the lambs vaccinated with peGn, pscDEC-eGn and pscCD11c-eGn. a A PCA plot of the clinical, virological and immune response data (=PCA variables) of vaccinated and non-vaccinated sheep is depicted, with each sheep represented as a dot in a specific color according to its group assignment: PC1 explains 36.82% of the total variation of the data between sheep and PC2 explained a further 18.21% of the variation. The vaccination regimen is indicated by a distinct color (red for peGn, blue for pscDEC-eGn, green for pscCD11c-eGn). b Thirty-one individual input variables were loaded (see Materials and methods) and the most relevant ones are indicated on the figure for clarity: viral RNA AUC, pfu AUC, individual clinical scores, integrated temperature measurements, T-cell responses at week 12, anti-eGn IgG OD signals (AbeGn) at 0, 5, 6 and 10 dpc, anti-N IgG OD signals (AbN) at 8 and 10 dpc, NAb titers (NAb) at 10 dpc and anti-RVFV IgG OD signals (AbRVFV) at 0, 5, 6 and 10 dpc. c Correlation analyses between the immune response data versus the clinical and virological data. For each vaccinated lamb, the anti-eGn and anti-RVFV IgG OD signals at 0, 5 and 6 dpc, the NAb titers at 10 dpc and the anti-N IgG OD signals at 5, 6 and 10 dpc were subjected to bilateral Spearman's correlation analysis against the individual clinical scores, the viral RNA AUC and the pfu AUC data. The statistically significant negative correlations are indicated in red (*p < 0.05; **p < 0.01) and the r coefficient is indicated
