Fig. 3

T cell cytokine profiles from whole blood ICS for Cohorts 1 and 2. Frequencies of ID93-specific CD4 T cells co-expressing different cytokines are shown for Cohorts 1 and 2 as measured by intracellular cytokine staining of ID93-stimulated whole blood with unstimulated values subtracted. The bar shows the median frequency, the box shows the interquartile range, and the whiskers show the maximum and minimum values. Vaccinations were given on Days 0, 28, and 56. a Combined data for 2 μg and 10 μg ID93 recipients, b 2 μg ID93 + 2 μg GLA-SE, c 10 μg ID93 + 2 μg GLA-SE. Wilcoxon matched pairs signed rank test to compare frequencies of ID93-specific cytokine response between Day 0 and Day 42 timepoints. d Proportions of ID93-specific CD4 T cells co-expressing different cytokines are shown for Cohort 1 and 2 subjects administered 2 μg ID93, 10 μg ID93, 2 μg ID93 + 2 μg GLA-SE, or 10 μg ID93 + 2 μg GLA-SE as measured by intracellular cytokine staining of ID93-stimulated whole blood with unstimulated values subtracted. Results are shown for Day 70 (2 weeks post third vaccination). Due to low subject numbers, results for 2 μg ID93 and 10 μg ID93 were combined