Fig. 3
Protection of vaccinated AG129 mice against lethal challenge with ZIKV strain SL1602. AG129 mice were vaccinated (blue squares, n = 10) with 1 × 104 PFU of YF-ZIKprM/E or sham-vaccinated (red circles, n = 10). Weight a and time to euthanasia b were monitored after challenge with either 1 × 103 PFU or 1 × 105 PFU of ZIKV SL1602. All vaccinated mice were grouped irrespective of the challenge dose. See Supplementary Fig. 9c and d for details about individual groups. c Protection from viremia in vaccinated AG129 mice: AG129 mice that had either been vaccinated with 1 × 104 PFU of YF-ZIKprM/E (blue squares, n = 10) or that were sham-vaccinated (red circles, n = 10) were challenged with 1 × 105 PFU or 1 × 103 PFU of ZIKV SL1602 and viral RNA copy numbers were assessed at 3 days post challenge. All vaccinated mice were grouped irrespective of the challenge dose. See Supplementary Fig. 9c and d for details about individual groups. Sera from vaccinated mice collected pre-challenge served as controls. d AG129 mice were vaccinated with either 1 × 102 PFU, 1 × 104 PFU or 1 × 106 PFU of YF-ZIKprM/E (blue squares, n = 5 per group) and were challenged with 1 × 105 PFU of ZIKV SL1602 and ZIKV RNA copies in serum on day 3 post challenge were measured. Because of increased background values of qRT-PCR due to change in virus strain (MR766 to SL1602), values are reported as fold changed in RNA copies compared to serum RNA values of vaccinated mice prior to challenge. e Expression of cytokines 3 days post challenge: Vaccinated (closed squares, n = 5) and sham-vaccinated (closed circles, n = 5) mice were challenged with 1 × 105 PFU of ZIKV SL1602 and levels of IFN-γ, IL-18, IL-6, MCP-1, and other cytokines (Supplementary Table 3) were assessed 3 days post challenge. Data are presented as mean values with error bars indicating SEM. Mann–Whitney two-tailed test was used for statistical analyses. Asterisk indicates a significant difference *P-values < 0.05, **P-values < 0.01. Background (dotted line) represents average RNA copies of vaccinated mice before challenge or the limit of detection (L.O.D) of the assay
