Fig. 1

EV71 and CA16 antigen distribution in various tissues and pathological features. Immunohistochemical observation of the EV71 and CA16 antigens after administration via a intradermal (ID) and b intramuscular (IM) routes at 12 h and 24 h post inoculation. c EV71 viral RNA and d CA16 viral RNA in local tissues of three groups. Pathological observation of e intradermal (ID) tissues and f intramuscular (IM) tissues. Scale bar is 100 µm. Data are representatives of three independent experiments (error bars represent SD).; Statistical significance were assessed by unpaired t tests (*p < 0.01, **p < 0.001)