Fig. 3
Relationships between the EV71 or CA16 antigen and ILC1 cells in local tissues post inoculation. a Representative confocal fluorescence images of EV71 expression (purple), GATA3 (green) and NKp46 (red) after intradermal (ID) or intramuscular (IM) administration of EV71 antigen at 12 and 24 h post inoculation. c Representative confocal fluorescence images of CA16 expression (purple), GATA3 (green) and NKp46 (red) after intradermal (ID) or intramuscular (IM) administration of the CA16 antigen at 12 and 24 h post inoculation. e Representative confocal fluorescence images of EV71+CA16 expression (purple), GATA3 (green) and NKp46 (red) at 12 and 24 h post inoculation of the EV71+CA16 antigens via the intradermal (ID) or intramuscular (IM) route. Statistical analysis of colocalization with b EV71, d CA16, and f EV71+CA16 cells. g Colocalization rates of ILC1, ILC2, and ILC3 cells after intradermal (ID) or intramuscular (IM) inoculation. Representative fluorescent cells in the white rectangle are shown at ×20 magnification after confocal microscopy. Red scale bar is 100 µm, white scale bar is 5 µm. Data are representatives of three independent experiments (error bars represent SD). Statistical significance were assessed by unpaired t tests (***p < 0.0001)
