Fig. 3
Signatures associated with nAb responses in YF-17D vaccinees. a nAb titers from the top three and bottom three responders for which microarray data were available in the cohort. Significant differences are determined using a two-tailed paired t test. *p < 0.05. b, c Top five pathways significantly enriched in comparing the transcriptomic signatures at 3 days post-YF-17D vaccination for three high responders and three low responders using GSEA Hallmark database (b) and GSEA Immunologic signature database (c). d Correlation plots between the log2 concentration of cytokines in the serum at 7 days post vaccination and the PRNT50 titer against YF-17D at 1-month post vaccination. One dot represents one individual. The three high responders and three low responders used in the GSEA analysis are highlighted in red and blue, respectively. The black dots represent the remaining individuals in the cohort whose responses were in between. A list of all cytokines analyzed is shown in Supplementary Fig. 7c. e Proposed model of immune signatures leading to high nAb responses to flavivirus infection
