Fig. 3
Functional analyses of murine antisera. a, b Hemagglutination inhibition (HI) titers against the indicated H3N2 viruses carrying hemagglutinin and neuraminidase of A/Hong Kong/4801/2014 (HK2014), A/Perth/16/2009 (Perth 2009), A/Philippines/2/1982 (Phi 1982), and A/Hong Kong/1/1968 (HK1968). a shows data for pooled sera from 15 mice measured in triplicates, b shows data for individual sera. The horizontal bars show the geometric mean values and the dashed lines the limit of detection. Statistical significance in b was inferred by performing analysis of variance with Newman–Keuls posttest on log-transformed values with ***P ≤ 0.001. c Microneutralization endpoint titers determined with HK2014 virus using pooled sera (n = 15 mice) measured in triplicates. The horizontal bars show the geometric mean values and the dashed lines the limit of detection. d, e In vitro antibody-dependent cellular cytotoxicity (ADCC) activity using MDCK cells infected with HK2014 virus (d) or with HK1968 virus (e). Data points represent mean ± SD of pooled sera from 15 mice measured in triplicates
