Table 1 Melting temperature (Tm) of wild-type and variant epsilon toxins

From: Clostridium perfringens epsilon toxin vaccine candidate lacking toxicity to cells expressing myelin and lymphocyte protein

Protein

Mean Tm (°C) ± SD

Wild-type epsilon toxin

67.4 ± 1.45

Y30A-Y196A

60.6 ± 0.15

Y30A-Y196-A168F

62.0 ± 0.22

Y30A-Y196A-H149A

60.9 ± 2.28

Y30A-Y196A-V166A

59.9 ± 0.47

Y30A-Y196A-V72F

59.5 ± 0.10

Y30A-Y196A-F92A

58.2 ± 0.09

Y30A-Y196A-H149A-A168F

57.4 ± 0.02

  1. Thermostability of proteins was determined by the Boltzmann method using the Protein Thermal Shift software (Applied Biosystems). Results represent the mean and standard deviation (SD) of measurements from duplicate samples
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