Fig. 2
From: Safety and efficacy of ChAdOx1 RVF vaccine against Rift Valley fever in pregnant sheep and goats
Vaccine immunogenicity and efficacy in pregnant ewes and does. Rectal temperatures, serological responses (RVFV VNT50 titres, anti-N ELISA) and viraemia following vaccination and challenge of pregnant ewes and goats are shown. Data in a, c, e and g are from pregnant ewes, whilst data in b, d, f and h are from pregnant does. Vaccination and challenge were performed as shown in Fig. 1. The black arrows represent the day of challenge. Anti-N ELISA titres (e and f) are expressed as percentage inhibition calculated as ratio of the optical densities (OD) of the sample and the OD of the negative control (% S/N) as per manufacturer’s instructions. All values lower than 40% are considered positive, between 40% and 50% are considered doubtful and above 50% are considered negative. The dotted lines represent the 40% and 50% inhibition. Samples that tested negative for viraemia are depicted at the detection limit of the RT-qPCR assay (1.3 log10 RNA copies/ml). All data are depicted as means and standard errors. p Values from Mann–Whitney U test comparing pre-challenge VNT50 titres (as measured on day 21; c and d) and viraemia levels at 3 days post-challenge (g and h) between mock- and ChAdOx1 RVF-vaccinated animals are shown; ***p < 0.001
