Fig. 4
From: Safety and efficacy of ChAdOx1 RVF vaccine against Rift Valley fever in pregnant sheep and goats
Histopathological analysis of sheep placentomes. Histopathological examination of placentomes of representative sheep from the ChAdOx1 RVF and mock-vaccinated groups are shown. The vaccine allocation and foetus numbers are indicated for each panel; these correspond to foetus and ewe numbers in Fig. 3. For each panel, the letters represent: a H&E staining of the placentome, b IHC staining with RVFV mAb 4-D4, c staining with Alizarin Red showing foci of calcium deposits, d same specimen as c but visualized under polarized light, e high magnification of H&E staining and f high magnification of IHC staining. Notice the calcium deposits (arrows in c and d, right column), haemorrhages and necrotic and calcified epithelium (asterisk and arrows in e, right column) in the mock-vaccinated group. Scale bars = 5000 μm (a–d) and 100 μm (e and f)
