Fig. 2: Reactivity of GP VLP-immunized rabbit sera against full-length LASV GP. | npj Vaccines

Fig. 2: Reactivity of GP VLP-immunized rabbit sera against full-length LASV GP.

From: Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity

Fig. 2

Immunofluorescence microscopy of a MDCK II cells stably expressing LASV GP or b Vero E6 cells transiently transfected with plasmids encoding LASV GP. For the staining of native antigens, the cells were incubated with either the pre-immune rabbit sera (D0 samples) or rabbit immune sera after final bleed (D77 samples) before fixation with 4% PFA. For the staining of fixed antigens, the cells were treated with 4% PFA and permeabilized and then incubated with indicated rabbit sera. Anti-rabbit Alexa Fluor 488 was used as a secondary antibody, and nuclei were visualized by DAPI staining. Merged images of LASV GP and nuclear staining are shown. Scale bars, 20 μm. c Immuno-EM of stable transfected (MDCK II LASV GPC cells) and nontransfected MDCK II cells. For immunogold labeling of GP, the cells were aldehyde fixed, pelleted, and embedded in LR White, and ultrathin sections were incubated with rabbit serum (Rb#347_D77 or Rb#350_D77) diluted 1:20, followed by protein A conjugated to 6 nm colloidal gold. Scale bars, 250 μm.

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