Fig. 6: Induction of robust neutralizing antibody titers requires several immunizations. | npj Vaccines

Fig. 6: Induction of robust neutralizing antibody titers requires several immunizations.

From: Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity

Fig. 6

a Geometric mean neutralizing antibody titers against VSVΔG/LASVGP at 0, 28, 49, 70, and 77 days are shown (n = 1). The initial dilution was 1:16 and was therefore denoted as a titer of eight for GMT calculation. b ELISA of total IgG response against LASV GP1 or GP2 of 1:200 dilution of rabbit sera collected at indicated time points is shown. Error bars are representative of the SD of OD 450 nm values (duplicate measurement of two independent experiments). The cut-off for a positive antibody response is indicated as a dashed line, calculated as the median of the pre-immune sera (D0) + 10%. c Immunofluorescence microscopy using MDCK II cells stably expressing LASV GP demonstrates the recognition of GP by serum antibodies collected from immunized rabbits at indicated time points post-priming. An unrelated rabbit serum was used as a negative control, while α231 and α3 were used as a positive control. All sera were diluted 1:200. Anti-rabbit Alexa Fluor 568 was used as a secondary antibody, and nuclei were visualized by DAPI staining. Merged images of GP and nuclear staining are shown. Scale bars, 200 µm.

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