Fig. 8: Purification and characterization of IgG preparations. | npj Vaccines

Fig. 8: Purification and characterization of IgG preparations.

From: Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity

Fig. 8

a Using protein A affinity chromatography, IgG was isolated from rabbit sera collected 1 week after the final boost (D77). For IgG purification analysis, 3 μg of total protein from each preparation was separated by SDS-PAGE and stained with SYPRO Ruby protein gel staining. Lanes 1 and 3: initial antiserum; lanes 2 and 4: elution of protein A affinity chromatography. b Neutralizing antibody response against VSVΔG/LASVGP of initial antisera (D77 samples) and affinity-purified IgG (D77+ samples). Bars represent the mean, and error bars indicate the standard deviation of GMT values of three independent experiments. c Detection of α-LASV GP1 or GP2 antibodies using ELISA. The values shown represent the means of the reciprocal serum dilutions. Error bars are representative of the SD of OD 450 nm values (duplicate measurement of three independent experiments).

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