Fig. 4: JEV-ΔNS1 is highly safe in mice. | npj Vaccines

Fig. 4: JEV-ΔNS1 is highly safe in mice.

From: A replication-defective Japanese encephalitis virus (JEV) vaccine candidate with NS1 deletion confers dual protection against JEV and West Nile virus in mice

Fig. 4

a, b Neuroinvasiveness tests for WT and JEV-ΔNS1 viruses in mice. Four-week-old C57BL/6 female mice (n = 5 for each group) were i.p. inoculated with 106 and 107 IU of WT JEV or 107 IU of JEV-ΔNS1. The mortality (a) and weight loss (b) were monitored for 21 days. c, d Neurovirulence tests for WT and JEV-ΔNS1 viruses in mice. Three-week-old ICR female mice (n = 5 for each group) were i.c. inoculated with 10 IU of WT JEV or 7.5 × 106 IU of JEV-ΔNS1. The mortality (c) and weight loss (d) were monitored for 21 days. Kaplan−Meier survival curves were analyzed by the log-rank test and the asterisks denote statistical differences between the indicated groups. *p < 0.05; ***p < 0.001. Two independent experiments were performed, and data from one experiment are presented.

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