Fig. 1: SARS-CoV-2 S-specific T cell responses following ChAdOx1 nCoV-19 prime-only and prime-boost vaccination regimens in mice and pigs. | npj Vaccines

Fig. 1: SARS-CoV-2 S-specific T cell responses following ChAdOx1 nCoV-19 prime-only and prime-boost vaccination regimens in mice and pigs.

From: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

Fig. 1

Inbred BALB/c (n = 5) and outbred CD1 (n = 8) were immunised on day 0 and 28 with ChAdOx1 nCoV19 (Prime-boost) or ChAdOx1 nCoV19 on day 28 (Prime-only); pigs (n = 3) were immunised with ChAdOx1 nCoV-19 on days 0 and 28 (Prime-boost), or only on day 0 (Prime-only). To analyse SARS-CoV-2 S-specific T cell responses, all mice were sacrificed on day 49 for isolation of splenocytes and pigs were blood sampled longitudinally to isolate PBMC. Following stimulation with SARS-CoV-2 S-peptides, responses of murine splenocytes a and porcine PBMC c were assessed by IFN-γ ELISpot assays. Using flow cytometry, CD4+ and CD8+ T cell responses were characterised by assessing expression of IFN-γ, TNF-α, IL-2, IL-4 and IL-10 (mice; b) and IFN-γ, TNF-α, IL-2 and IL-4 (pigs; d). Each data point represents an individual mouse/pig with bars denoting the median response per group/timepoint. Data were analysed by ANOVA and statistically significant differences between vaccine groups are indicated: *p < 0.05.

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