Fig. 5: Cryo-EM structure reconstruction of HPV16 in complex with Fab H16.001. | npj Vaccines

Fig. 5: Cryo-EM structure reconstruction of HPV16 in complex with Fab H16.001.

From: Structural characterization of a neutralizing mAb H16.001, a potent candidate for a common potency assay for various HPV16 VLPs

Fig. 5

(a) Representative 2D classification averages of the immune-complex H16-001. (b) Central section of density map of the immune-complex H16-001 (on the twofold axis, with symmetry axes indicated in black). Scale bar = 30 nm. c The iso-contoured view of cryo-EM density map of the immune-complex H16-001 (radially colored) is shown along the icosahedral twofold axes. Icosahedral twofold, threefold, and fivefold axes are indicated by black symbols. (d) Closed-up view of the boxed area in (c). The number indicates the six L1 monomers in an icosahedral asymmetric unit. The pentagon and hexagon symbols represent 5-coordinated and 6-coornidated pentamer, respectively.

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