Fig. 2: Whole-blood transcriptomic response of control cows during the course of an E. coli mastitis. | npj Vaccines

Fig. 2: Whole-blood transcriptomic response of control cows during the course of an E. coli mastitis.

From: Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis

Fig. 2

Differentially expressed genes (DEGs) between times were assessed using Deseq2. a A principal component analysis on the top 500 most differentially expressed genes of control cows at 0, 12, and 40 h post infection (hpi) shows that the expression profile of blood cells changed according to the time during the E. coli mammary infection. A Venn diagram presents the number of DEGs between time points, and the number of genes in common between the two comparisons (between 12 and 0 hpi and between 40 and 0 hpi) (b). Volcano plots were used for determining DEGs in the CONT group between 12 and 0 hpi (c), and 40 and 0 hpi (d). Thresholds were arbitrarily established for a q value < 0.01 and a log2FoldChange > 2 and genes above these thresholds are depicted in red dots. e A Heatmap of the top 500 DEGs during infection in control group is presented using Euclidian distribution analysis. Results of the functional analysis with ToppFun is presented as a dendrogram showing the relationship among biological functions and blocks of commonly regulated genes.

Back to article page