Fig. 6: Gene expression profile of CD4 T cells isolated from the mammary tissue of control and challenged quarters from locally immunized and control cows. | npj Vaccines

Fig. 6: Gene expression profile of CD4 T cells isolated from the mammary tissue of control and challenged quarters from locally immunized and control cows.

From: Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis

Fig. 6

a Experimental scheme with immunization and challenge phases. DEGs were assessed between four conditions corresponding to challenged and control quarters in CONT and IMM cows. b A heatmap showing the top 500 DEGs according to the conditions is presented. Functional analysis performed with ToppGene is presented as a dendrogram of functions in each block of correlated genes (p value < 0.05). c Gene network of the DEGs in CD4 T cells between the infected IMM and infected CONT conditions is presented and shows the relationship with IL-17 cytokine. d Tissue IL-17A and CCL2 production in non-infected and challenged quarters of CONT and IMM cows was determined on a pre-defined amount of tissue homogenate with a Milliplex assay at 16 hpi. Individual values are shown for n = 3 cows per group.

Back to article page