Fig. 2: Optimisation of the NHP direct PBMC MGIA.

Samples were collected from 12 Rhesus macaques from Study 2, six of which were naïve unvaccinated controls (green boxes), and six of which received BCG vaccination by the intradermal (ID) route (blue boxes). The MGIA was performed using PBMC from baseline (circles) and 8 weeks post-BCG vaccination (triangles) according to the original ‘in-tube protocol’ co-culturing in 2 ml screw-cap rotating tubes with (a) 1 × 10⁶ PBMC or (b) 5 × 10⁶ PBMC and a BCG inoculum of 500 CFU. Points represent individual animals with the mean of two co-culture replicates (a) or a single co-culture (b). Normalised mycobacterial growth is equal to (log₁₀ CFU of sample – log₁₀ CFU of inoculum control). Boxes indicate the median value with the interquartile range and whiskers indicate the minimum and maximum values. A one-way ANOVA with Sidak’s multiple comparisons test was performed where *p-value < 0.05.