Fig. 6: Mapping of linear B-cell epitopes across MSP1. | npj Vaccines

Fig. 6: Mapping of linear B-cell epitopes across MSP1.

From: Immunization with full-length Plasmodium falciparum merozoite surface protein 1 is safe and elicits functional cytophilic antibodies in a randomized first-in-human trial

Fig. 6

Sera from vaccinees (1:1000), two malaria semi-immune adults from Burkina Faso (1:1000), and two MSP1 immunized rabbits (1:10,000) were applied to a custom-made MSP1 peptide microarray chip consisting of 1706 oligomeric peptides of 15 amino acids with a peptide-to-peptide overlap of 14 amino acids. Each peptide was printed in duplicate on the chip. As secondary antibodies the appropriate DyLight680 conjugated anti-IgG (Fc) antibodies were used. The fluorescence intensity landscapes across MSP1 are shown for each sample. Relevant epitopes have been highlighted. Controls comprise the placebo and the GLA-SE vaccinees. For orientation, the structural organization of MSP1 is shown below the fluorescence profiles. Full details of epitopes and fluorescence intensities are provided in the Supplementary Table 2.

Back to article page