Fig. 7: Association of GIA activity with MSP1 epitopes. | npj Vaccines

Fig. 7: Association of GIA activity with MSP1 epitopes.

From: Immunization with full-length Plasmodium falciparum merozoite surface protein 1 is safe and elicits functional cytophilic antibodies in a randomized first-in-human trial

Fig. 7

Major epitopes putatively associated with GIA activity as defined by LOD scores > 2.5 (<p = 0.001) for at least three consecutive peptides are highlighted. The GIA activity underpinning the correlation are depicted in Fig. 5a, with sera from MSP1-immunized rabbits and semi-immune adults from Burkina Faso showing GIA activity, whereas sera from vaccinees being GIA inactive. Epitopes that map to MSP1 processing sites16,83 are indicated in blue, with the cleavage site being pointed out by an arrow. Note that three processing sites were detected between the p38/p42 junction: the canonical cleavage site (TGEAISV) and two adjacent alternative sites. Another epitope includes a lysine (highlighted in red) that is posttranslationally modified by acetylation. The correlation was determined using a Pearson function followed by a Bonferroni correction and conversion of the resulting p-values into a LOD score (logarithm of odds). The confidence line as defined by p < 0.001 is indicated. For orientation, the structural organization of MSP1 is shown below the graph. Full details of epitopes and LOD scores are provided in the Supplementary Table 2.

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