Fig. 3: Pfs230C1, but not Pfs25, is taken up by APCs without particleization and is effectively adjuvanted by Montanide ISA720.
From: Antibody response of a particle-inducing, liposome vaccine adjuvant admixed with a Pfs230 fragment
Anti-Pfs230C1 and anti-Pfs25 IgG ELISA (a) and SMFA (b) with indicated antigen adjuvanted with CoPoP/PHAD or ISA720. c RAW264.7 macrophage uptake of fluorophore-labeled Pfs25 or Pfs230C1 with indicated adjuvant assessed as mean fluorescence intensity (MFI) using flow cytometry. d Lymph node uptake of antigens into APCs following intramuscular injection. e Uptake ratio of antigens adjuvanted with CoPoP over ISA720 in lymph nodes. ELISA experiment in a were performed with n = 10 independent mice and lines show geometric mean. SMFA experiments in (b) were performed with n = 20 mosquitoes and lines show arithmetic mean. Bar graphs show mean±std. dev. for n = 3 independent experiments. Pfs25 asterisks in a, b, d and e indicate data re-use that was previously reported in ref. 18. For (a), the titer comparison between CoPoP/PHAD and ISA720 with indicated proteins, log-transformed data was compared with Student's t test. For (c), one-way ANOVA followed by Tukey’s comparison was used. For e, the comparison between CoPoP/ISA720 ratio uptake in APC was compared with Student's t test. *p < 0.05, **p < 0.01; ****p < 0.0001.
