Table 1 Number of sequence reads detected for each of the spiked viruses at the different spiking levels in the viral vaccine crude harvest.
From: Sensitivity and breadth of detection of high-throughput sequencing for adventitious virus detection
Virus | Spiking level (GC mL−1) | |||||
|---|---|---|---|---|---|---|
105 | 104 | 103 | 103 | 102 | 102 | |
Total raw reads | 291,341,932 | 371,184,528 | 365,131,932 | 333,373,964 | 437,070,286 | 332,181,730 |
NIH | ||||||
Adenoviridae | ||||||
Adenovirus 5 | 14,382 | 2284 | 264 | 193 | 34 | 34 |
Adenovirus 41 | 1154 | 164 | 12 | 16 | 11 | 6 |
Flaviviridae | ||||||
Bovine Viral Diarrhea Virus | 95,666 | 6306 | 776 | 926 | 68 | 57 |
Herpesviridae | ||||||
Herpes Simplex Virus Type 1 | 1366 | 200 | 40 | 23 | 6 | 2 |
Simian Cytomegalovirus | 49,266 | 9386 | 1170 | 676 | 116 | 87 |
Orthomyxoviridae | ||||||
Influenza A Virus | 16,553 | 1266 | 83 | 166 | 8 | 3 |
Paramyxoviridae | ||||||
Bovine Parainfluenza Virus Type 3 | 48,578 | 3548 | 321 | 410 | 24 | 32 |
Measles Morbillivirus | 12,571 | 922 | 87 | 105 | 0 | 10 |
Mumps Virus | 9041 | 524 | 86 | 46 | 10 | 2 |
Picornaviridae | ||||||
Coxsackievirus A16 | 351 | 27 | 1 | 0 | 3* | 0 |
Coxsackievirus B3 | 2036 | 157 | 10 | 18 | 9 | 8 |
Echovirus 11 | 2539 | 140 | 4 | 14 | 5* | 1 |
Rhinovirus 2 | 1259 | 62 | 6 | 20 | 0 | 4 |
Polyomaviridae | ||||||
Simian Virus 40 | 528 | 88 | 47 | 15 | 10 | 3* |
Rhabdoviridae | ||||||
Vesicular Stomatitis Virus | 13,120 | 1071 | 102 | 157 | 27 | 12 |
Togaviridae | ||||||
Rubella Virus | 4610 | 391 | 76 | 51 | 4 | 2 |
SP | ||||||
Bornaviridae | ||||||
Human Borna Disease Virus | 341 | 6 | 1 | 0 | 0 | 0 |
Coronaviridae | ||||||
Bovine Coronavirus | 7447 | 473 | 48 | 46 | 5* | 2 |
Herpesviridae | ||||||
Human Cytomegalovirus | 162,008 | 28,013 | 2733 | 2657 | 305 | 252 |
Parvoviridae | ||||||
Minute Virus of Mice | 319 | 48 | 12 | 10 | 0 | 0 |
Porcine Parvovirus | 146,720 | 20,668 | 2412 | 2609 | 397 | 238 |
Reoviridae | ||||||
Reovirus Type 3 | 11 | 0 | 0 | 0 | 0 | 0 |
