Table 2 Repeatability of the number of reads detected by the HTS test across 3 replicate datasets in the viral vaccine crude harvest.

Spiked virus	10⁴ GC mL⁻¹ replicate 1	10⁴ GC mL⁻¹ replicate 2	10⁴ GC mL⁻¹ replicate 3	Mean number of reads at 10⁴ genome copies per mL	Standard deviation	% Coefficient of variation
NIH
Adenoviridae
Adenovirus 5	2284	1792	2570	2215	394	18
Adenovirus 41	164	120	176	153	29	19
Flaviviridae
Bovine Viral Diarrhea Virus	6306	18,680	21,484	15,490	8076	52
Herpesviridae
Herpes Simplex Virus Type 1	200	255	309	293	33	11
Simian Cytomegalovirus	9386	3,760	5,080	6,075	2942	48
Orthomyxoviridae
Influenza A Virus	1266	21	33	5,408	3953	73
Paramyxoviridae
Bovine Parainfluenza Virus Type 3	3548	8760	10,795	7701	3738	49
Measles Morbillivirus	922	1950	1778	1550	551	36
Mumps Virus	524	3602	5741	3289	2623	80
Picornaviridae
Coxsackievirus A16	27	112	53	64	44	69
Coxsackievirus B3	157	969	986	704	474	67
Echovirus 11	140	847	1,854	947	861	91
Rhinovirus 2	62	238	197	166	92	55
Polyomaviridae
Simian Virus 40	88	62	75	75	13	17
Rhabdoviridae
Vesicular Stomatitis Virus	1071	9257	13,396	7908	6272	79
Togaviridae
Rubella Virus	391	560	796	582	203	35
SP
Bornaviridae
Human Borna Disease Virus	6	403	447	285	243	85
Coronaviridae
Bovine Coronavirus	473	1369	1813	1218	683	56
Herpesviridae
Human Cytomegalovirus	28,013	139,737	66,743	162,203	108,453	67
Parvoviridae
Minute Virus of Mice	104	130	1813	94	42	45
Porcine Parvovirus	20,668	54,941	15,775	30,461	21,341	70
Reoviridae
Reovirus Type 3	0	120	49	56	60	107

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