Table 4 Comparison of the sensitivity in a viral vaccine crude harvest versus sensitivity of the NIH in vivo tests.
From: Sensitivity and breadth of detection of high-throughput sequencing for adventitious virus detection
NIH virus | In vivo LOD (TCID50, as described previously)9 | Sensitivity of the in vivo assay (GC, determined by Clean Cell) | Sensitivity of the HTS assay (GC) |
|---|---|---|---|
Adenoviridae | |||
Adenovirus 5 | NT | NT | 90 |
Adenovirus 41 | NT | NT | 9000 |
Flaviviridae | |||
Bovine Viral Diarrhea Virus | Not detected | ≥3.39 × 108 | 90 |
Herpesviridae | |||
Herpes Simplex Virus Type 1 | 100 | 3.4 × 104 | 900 |
Simian Cytomegalovirus | NT | NT | 90 |
Orthomyxoviridae | |||
Influenza A Virus | 10−4 | 5.46 × 10−4* | 90* |
Paramyxoviridae | |||
Bovine Parainfluenza Virus Type 3 | Not detected | ≥8.67 × 107 | 90 |
Mumps Virus | Undiluted | 1.12 × 109 | 900 |
Measles Morbillivirus | Not detected | ≥4.00 × 108 | 900 |
Picornaviridae | |||
Coxsackievirus A16 | 105 | 3.03 × 108 | 9000 |
Coxsackievirus B3 | 1 | 3.0 × 104 | 900 |
Echovirus 11 | Not detected | ≥6.29 × 109 | 900 |
Rhinovirus 2 | NT | NT | 9000 |
Polyomaviridae | |||
Simian Virus 40 | NT | NT | 9000 |
Rhabdoviridae | |||
Vesicular Stomatitis Virus | 10−4 | 1.28 × 10−4* | 90* |
Togaviridae | |||
Rubella Virus | Not detected | ≥2.45 × 107 | 900 |
