Fig. 3: Anti-M cellular immune responses are specific to defined regions. | npj Vaccines

Fig. 3: Anti-M cellular immune responses are specific to defined regions.

From: A CCHFV DNA vaccine protects against heterologous challenge and establishes GP38 as immunorelevant in mice

Fig. 3

Groups of 9 female C57BL/6 mice were vaccinated with 50 μg CCHFV-M10200 or empty vector on days 0, 21, and 42 by IM-EP, and then euthanized on day 49 for splenocyte T cell analysis. Splenocytes from individual mice were re-stimulated with pooled peptides derived from the CCHFV-IbAr 10200 M-segment. Anti-CCHFV-M specific (a) IFN-γ+ and (b) IL-2+ T cells were quantified by ELISPOT. The corresponding region of the peptide pools to the M-segment is shown below each graph. Percentage of (c) IFN-γ+ and (d) IL-2+ T cells responding to each peptide pool. Data are the group mean averages ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ***p < 0.0001. p values were determined by two-way ANOVA with Sidak’s multiple comparison test with a 95% confidence interval.

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