Fig. 5: Inhibition of VLA-4 increases Nβ/Nα ratio.

Contour plot composition of peritoneal immune infiltrate after NYVAC-C Δ3 infection. Proportion of TCR⁺CD4⁺ and TCR⁺CD8⁺ T cells, CD11b⁺ Ly6G⁺ neutrophils divided by SSC and FSC in Nα and Nβ, CD11b⁺ Ly6G⁻ Ly6C⁺ monocytes (M), B220⁺ NK1.1⁻ B cells, NK1.1⁺ NK cells, MHCII⁺ CD11c⁺ dendritic cells (DC) and F4/80⁺ MHCII⁺ macrophages (MΦ) were measured at 6 h post infection (a). Total numbers of NK, B, CD4, CD8, DC, MΦ, M, N, Nα, and Nβ from PEC (b) and spleen (c) of NYVAC-C Δ3 infected and IgG2b or anti-VLA4 antibody-treated mice. Mice were injected with IgG2b isotype or anti-VLA4 antibody 16 h before- and 1 h post infection. Nβ/Nα ratio from PEC (d), spleen (e), and blood (f) of infected and IgG2b or anti-VLA4 antibody-treated mice. Graphs show mean; each point represents an individual mouse. Data are representative of two independent experiments. *P < 0.05.