Fig. 3: Efficacy of T-LAIV following challenge with A/Texas/50/2012 (H3N2).
a Study design. Four to six 12-month-old ferrets were intranasally vaccinated with 1 × 107 EID50 liquid LAIV, T-LAIV, T-LAIV + Advax adjuvant, or PBS. Ferrets were challenged with antigenically matched influenza virus (A/Texas/50/2012 (H3N2)) 4 weeks post-vaccination. Nasal washes were collected post-vaccination and post-challenge (blue arrows). Whole blood was collected pre-vaccination, post-vaccination/pre-challenge, and post-challenge (red arrows). b Weight change (percent) post-challenge with A/Texas/50/2012 (H3N2). Immunized ferrets were challenged intranasally with 1 × 107 PFU A/Texas/50/2012 (H3N2) and weights were recorded daily for 7 days post-inoculation. Percent weight change was compared to PBS challenge group. Two-way ANOVA with Dunnett’s multiple comparisons test. Error bars represent mean ± 95% CI. *p < 0.05. n = 4–6. c Viral loads detected in ferret nasal washes post-challenge with A/Texas/50/2012 (H3N2). Virus titer was determined by titration in embryonated chicken eggs. Kruskal–Wallis test with a post hoc Dunn’s multiple comparisons test. *p < 0.05, **p < 0.01. Error bars indicate mean ± 95% CI. n = 4–6.
