Fig. 7: Anti-FEN antibody affinity, IgG2a, and IgA isotypes contributed to protection from fentanyl challenge in dmLT and LTA1 immunized animals. | npj Vaccines

Fig. 7: Anti-FEN antibody affinity, IgG2a, and IgA isotypes contributed to protection from fentanyl challenge in dmLT and LTA1 immunized animals.

From: Fentanyl conjugate vaccine by injected or mucosal delivery with dmLT or LTA1 adjuvants implicates IgA in protection from drug challenge

Fig. 7

Serum samples from fentanyl challenge experiments were further characterized for anti-FEN immunity. a IgG antibody binding affinity calculated as % IgG binding to FEN-TT in the presence of a chaotropic agent (week 6). b Compiled serum anti-FEN (FEN-TT) serum IgG1 (week 8), IgG2a (week 8), IgA (week 10) ELISA from tailbleeds. c Ratio of serum anti-FEN (FEN-TT) IgG1/IgG2a. (Zero values for IgG2a were replaced with a 1.) d Correlation between hotplate antinociception vs IgG2a or IgG binding affinity for 0.1 and 1 μg dmLT IM immunization groups only. e Correlation between brain fentanyl levels or tail flick antinociception %MPE vs anti-FEN serum IgA (week 10) for immunized groups. Bars at mean + s.e.m. with significance determined by ANOVA paired with Bonferroni post-hoc test as shown (*P < 0.05, **P < 0.01, ***P < 0.001). Spearman correlations P values and correlation coefficient (r) indicated on relevant graph.

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