Fig. 1: Bpm-specific antibody responses induced by CLH001 and PBK001 vaccination. | npj Vaccines

Fig. 1: Bpm-specific antibody responses induced by CLH001 and PBK001 vaccination.

From: Antigen-specific antibody and polyfunctional T cells generated by respiratory immunization with protective Burkholderia ΔtonB Δhcp1 live attenuated vaccines

Fig. 1

a Experimental design and timeline of prime and boost regimen of PBS, CLH001, and PBK001 vaccination (i.n.), aerosol challenge and sample collection. b Serum anti-Bpm IgG antibody, c serum anti-Bpm IgM antibody, d serum anti-Bpm IgA antibody, e lung anti-Bpm IgG antibody, f lung anti-Bpm IgM antibody, g lung anti-Bpm IgA antibody. The sera (n = 10/group) and lung homogenate supernatant (n = 10/group) were collected two weeks after the 2nd boost. ELISA was performed by incubating diluted serum and lung homogenate supernatants (2-fold dilution) with irradiated Bpm K96243 whole cell lysates. The graphs represent mean ± SEM of OD450 value. Data were analyzed using one-way ANOVA followed by Tukey test for multiple comparison. *p < 0.05 PBS vs. CLH001; +p < 0.05 PBS vs. PBK001. The figure a was created using BioRender (https://biorender.com/).

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