Fig. 3: Respiratory immunization with CLH001 and PBK001 generates spleen and lung populations with cytokine recall to Bpm. | npj Vaccines

Fig. 3: Respiratory immunization with CLH001 and PBK001 generates spleen and lung populations with cytokine recall to Bpm.

From: Antigen-specific antibody and polyfunctional T cells generated by respiratory immunization with protective Burkholderia ΔtonB Δhcp1 live attenuated vaccines

Fig. 3

C57BL/6 mice were inoculated with PBS (control), CLH001, or PBK001. Bone marrow-derived dendritic cells (BMDC) were pulsed with 1 μg WCL of Bpm K96243 or BSA (10 ug) as mock antigen. Spleen and lung were harvested at 21 days post vaccination, and mononuclear cells were isolated from disrupted organs. BMDC were overlaid with organ mononuclear cells and cultured at 37 °C and 5% CO2. Culture supernatants were harvested after 72 h co-culture with mock- or Bpm-pulsed BMDC. The production of cytokines in spleen (a–d) and lung (e–h) was measured by ELISA. Data are representative of mean ± SEM from 10 mice/group. Group comparisons were analyzed using t-test or Mann–Whitney test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. = not significant).

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