Fig. 6: Intranasal immunization with CLH001 and PBK001 promotes a Th1 effector cytokine response and reduced bacterial burden upon Bpm infection. | npj Vaccines

Fig. 6: Intranasal immunization with CLH001 and PBK001 promotes a Th1 effector cytokine response and reduced bacterial burden upon Bpm infection.

From: Antigen-specific antibody and polyfunctional T cells generated by respiratory immunization with protective Burkholderia ΔtonB Δhcp1 live attenuated vaccines

Fig. 6

Bacterial burden in a lung, b liver, and c spleen of mice infected with Bpm K96243 were determined at 48 h after aerosol challenge. The symbols represent individual animals. The lines indicate the mean CFU/g of organ from each group. The proinflammatory cytokine/chemokine and Th1/Th2 effectors profiles in d, f serum and e, g lung of mice receiving PBS, CLH001, and PBK001 following 48 h exposure to Bpm K96243 via aerosol were measured using ELISA. Mean ± SEM plotted are representative of 5 animals. The significantly different values between non-vaccinated (PBS) and vaccinated groups were analyzed using t-test: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not significant.

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