Fig. 8: Analysis of histology indicates the CLH001 and PBK001 vaccination is associated with mild to moderate lung inflammation post vaccination.
The lungs (n = 3/group) of mice receiving PBS (a), CLH001 (b), or PBK001 (c) were collected 2 weeks after the 2nd boost. The lungs were placed in 10% formalin, fixed, paraffin-embedded, and stained with hematoxylin and eosin (H&E). The stained tissue sections were examined in a blind manner by a pathologist. The lungs of animals vaccinated with PBS was mostly non-remarkable except for some areas of mild interstitial inflammation. In lungs of CLH001 vaccinees, some areas of bronchovascular infiltrates (single arrow) were observed. The bronchovascular infiltrates were a more prevalent characteristic in lungs of mice vaccinated with PBK001, and frequent interstitial inflammation (double arrow) was also observed. Representative fields of view for lung tissues were taken at ×10 magnification. The scale bar represents 100 µm.
