Fig. 6: BAL from i.t.-AHc-immunized mice neutralized the toxicity of 100× i.p. LD50 or 500× i.p. LD50 before challenge via the i.p. route. | npj Vaccines

Fig. 6: BAL from i.t.-AHc-immunized mice neutralized the toxicity of 100× i.p. LD50 or 500× i.p. LD50 before challenge via the i.p. route.

From: Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice

Fig. 6

BoNT/A was incubated with BAL from AHc-immunized mice in PBS (1:1) for 30 min at room temperature. a, d BAL from dry powder formulation-immunized mice. b, e BAL from reconstituted powder formulation-immunized mice. c, f BAL from liquid formulation-immunized mice. Then mice were challenged with a–c 100× i.p. LD50 (n = 5) or d–f 500× i.p. LD50 (n = 10) of BoNT/A that had been preincubated with BAL from different groups. The animal survival rate was analyzed by Kaplan–Meier survival estimates. *P < 0.05.

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