Fig. 5: Analysis of HI antibodies in the serum of chickens vaccinated with rH9HA, rH9HA-CD83 scFv, and inactivated H9N2 virus. | npj Vaccines

Fig. 5: Analysis of HI antibodies in the serum of chickens vaccinated with rH9HA, rH9HA-CD83 scFv, and inactivated H9N2 virus.

From: Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza

Fig. 5

Groups of 7 days old chickens (n = 8) were immunised with 2.8, 28, and 49 µg of recombinant rH9HA-CD83 scFv equivalent to 2, 20, and 35 µg of recombinant rH9HA (equimolar concentration). Boost vaccination was given after 7 days post primary vaccination. The chickens were bled on day 6, 14, 21, and 28 posts primary vaccination. Antibody titres in sera were measured by haemagglutinin inhibition (HI) assay. The highest dilution of serum inhibiting the agglutination of RBCs by H9N2 virus (UDL01/08) was recorded. Data are presented as mean ± SD and analysed by one-way ANOVA followed by Tukey’s multiple comparison test. ****p < 0.0001 ***p < 0.001 **p < 0.01 *p < 0.05. ppv post primary vaccination.

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