Fig. 9: Challenge virus shedding profiles from the buccal cavity of vaccinated and PBS treated control chickens infected with H9N2 virus (UDL 01/08). | npj Vaccines

Fig. 9: Challenge virus shedding profiles from the buccal cavity of vaccinated and PBS treated control chickens infected with H9N2 virus (UDL 01/08).

From: Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza

Fig. 9

Groups of 7-day-old chickens (n = 7) were immunised with 35 µg of recombinant rH9HA-CD83 scFv equivalent to 25 µg of recombinant rH9HA (equimolar concentration). The second vaccine dose as boost was given on day 7 post primary vaccination. The chickens were challenged with 100 μl of 1 × 107 pfu/ml H9N2 virus (UDL 01/2008) after 7 days post boost vaccination. Some of the vaccinated and control birds were left unchallenged to serve as contacts. Swabs were taken from the buccal cavity until day 7 post infection and the amount of live virus particles recovered from swabs was titrated via plaque assays and presented as plaque-forming units (PFU)/ml. a Shedding profiles of each chicken from the buccal cavity at different days post infection are indicated by a single coloured dot. b The average shedding profile from the buccal cavity per group for directly infected birds. c The average shedding profile from buccal cavity per group for contact birds. Data are presented as mean ± SD for (a) and analysed by one-way ANOVA followed by Tukey’s multiple comparison test for (a) and by unpaired t-test for (b, c). For (b) the asterisks represent significant difference between rH9HA and rH9HA-CD83 scFv (direct) groups. ****p < 0.0001 ***p < 0.001 **p < 0.01 *p < 0.05. pi post infection.

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