Table 1 Mass Balance summary of ProC6C.

From: Preclinical development of a Pfs230-Pfs48/45 chimeric malaria transmission-blocking vaccine

Step

1 L Total protein (mg/L)

1 L ProC6C (mg/L)

5 L Total protein (mg/L)

5 L ProC6C (mg/L)

Harvest supernatant

22300a

30–35

22800a

30–40

Diafiltration/buffer exchange

680

30–35

645

38

Capture (Q)

390

25–27b

360

26–30b

HCP removal (Capture select)

40

18–22b

35

18–22b

Polishing (Q)

15

12–15c

12

10–12c

  1. aTotal protein is over-estimated due to the presence of Cysteine and Cystine in the medium.
  2. bAmount of ProC6C in the eluate.
  3. cAmount of ProC6C monomer in the eluate.
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