Fig. 2: Vaccination with HA clamp induces a neutralising and protective immune response in mice.
From: Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
A Schematic of vaccination groups, as well as the bleed and immunisation schedule for the H3 HA vaccination study. B Immunogenicity of the H3 vaccine antigens measured by ELISA and PRNT with serum collected on day 63. C Schematic of the vaccination groups for the H1 HA challenge study. D Immunogenicity of the H1 vaccine antigens measured by ELISA and PRNT from serum collected on day 62. Weight loss and survival curves of mice vaccinated with H1 HA proteins and challenged with E 102 PFU and F 5.5 × 103 PFU of A/Auckland/1/2009(H1N1pdm09) virus. G Virus titre in lung homogenates from mice infected with 102 PFU of virus at day 6 post infection. H Histopathology of mice infected with either 102 PFU (day 6 and 10) or 5.5 × 103 PFU (day 12). Data is presented as geometric mean with error bars representing SD. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001, determined using one-way ANOVA with Tukey’s multiple comparison post hoc test.
